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Arboriculture & Urban Forestry Online
Volume 34, Issue 5 — September 2008

A New and Improved Automated Technology for Early Sex Determination of Ginkgo biloba    (View PDF)

Vincent Echenard, François Lefort, Gautier Calmin, Robert Perroulaz, and Lassaad Belhahri

Abstract: Random amplified polymorphic DNA (RAPD) technique with male associated decamer primer S1478 was used to amplify DNA from 72 leaf samples collected from Ginkgo biloba trees with known sexual determinism in the canton of Geneva, Switzerland. This marker was found to be male-specific and was lacking in all female plants. Automated random polymorphic DNA analysis (ARPA), a new automated technology developed in the frame of this work, proved highly effective in distinguishing males and females with 100% efficiency and successful in male and female discrimination from a collection of young seedlings derived from a sexual cross. Our findings provide unambiguous evidence that ARPA combined with the male-associated decamer primer S1478 could be considered an efficient, rapid, and easy method to make an early sex determination in the dioecious tree Ginkgo biloba.


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