Influence of Coconut Milk Source on Host-Pathogen Interactions Between Ophiostoma Ulmi and Ulmus Selections
Subhash C. Domir, Lawrence R. Schreiber, and Steven M. Eshita
Abstract: This study compared the effects of two sources of coconut milk in modified Murashige and Skoog medium on growth of Ophiostoma ulmi on five selections of elm callus cultures. One medium (CMC) contained coconut milk from fresh coconuts; the other medium, (CMS), coconut milk obtained from Sigma Chemical Co. These studies showed that the coconut milk source affected fungal growth significantly, varying with elm selection and aggressiveness of the Ophiostoma ulmi isolate. An evaluation was made on production of secondary metabolites in callus cultures with each source of coconut milk. High performance liquid chromatography from Ulmus americana showed a higher level of those secondary metabolites using CMS medium when callus was from mature, DED-susceptible trees, but higher levels with CMC when callus was from DED-resistant trees.