Effects of Frequent Subculturing of Elm Callus Tissue on Growth of Ophiostoma Ulmi    (View PDF)

Subhash C. Domir and Lawrence R. Schreiber

Abstract: The effects of the frequency of subculturing on growth of the Dutch elm disease fungus, Ophiostoma ulmi, on elm callus cultures were examined. Callus tissues were generated from leaf tissue of susceptible American elm, resistant American elms, and resistant Siberian elm. Half the cultures was maintained at 22° C and subcultured at six-week intervals; the other half was maintained at 4° C and not subcultured. The callus cultures were inoculated either with an aggressive or nonaggressive isolate of O. ulmi. The data showed absence of correlation between fungal growth on callus tissue and frequency of subculturing. This lack of correlation is attributable to lack of homogeneity in callus tissue due to induction of somaclonal variation.

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https://doi.org/10.48044/jauf.1993.002