Determination of Parentage of Flowering Dogwood (Cornus Florida) Seedlings Using Dna Amplification Fingerprinting    (View PDF)

Malissa H. Ament, Mark T. Windham,and Robert N. Trigiano

Abstract: Open-pollinated seedlings of Cornus jlorida L. 'Cherokee Chief were tested to identify the pollen donor from a list of nearby C. florida trees using DNA amplification fingerprinting (DAF). Tissue was collected and DNA isolated from the maternal 'Cherokee Chief tree and possible pollen donors C. florida 'Cherokee Brave', 'Cherokee Daybreak', 'Cloud 9', 'Springtime', and 'Pygmy'. DNA was also isolated from C. florida 'Fragrant Cloud', 'Cherokee Princess', and 'Appalachian Spring'. Data from DAF were analyzed for similarities and differences between genetic makeup of seedlings and putative parents. Thirteen of 15 (87%) evaluated seedlings were the progeny of 'Cherokee Brave' and 'Cherokee Chief. Using open pollination, progeny of 2 red-bracted C. florida cultivars 'Cherokee Brave' and 'Cherokee Chief were obtained and subsequently verified using DAF.

Keywords: DNA amplification fingerprinting; cultivars; dogwood breeding; pollination.

https://doi.org/10.48044/jauf.2000.024